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Antibodies
Anti-CRISPR/Cas9 antibody, Mouse monoclonal
Anti-CRISPR/Cas9 antibody, Mouse monoclonal from MilliporeSigma
Be the first to write a review!
Citations:
(7)
Supplier Page
Supplier Page from
MilliporeSigma for
Anti-CRISPR/Cas9 antibody, Mouse monoclonal
MilliporeSigma's
Anti-CRISPR/Cas9 antibody, Mouse monoclonal
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: Blotting, Immunofluorescence, Immunoprecipitation, and Western Blot.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-CRISPR/Cas9 antibody, Mouse monoclonal
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
SAB4200701
Quantity
100µL, 25µL
Applications
immunoblotting, immunofluorescence, immunoprecipitation (IP)
Conjugate/Tag
Unconjugated
Target
CRISPR/Cas9
Isotype
IgG1
Clone
7A9-3A3
Host
mouse
Clonality
Monoclonal
Concentration
~1 mg/mL
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MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (7)
(1)
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two
Plant Methods
March 28, 2023
Jana Ordon, Niklas Kiel, Dieter Becker, Carola Kretschmer, Paul Schulze-Lefert, Johannes Stuttmann
nitrocellulose or PVDF
membranes
by tank
blotting
. Monoclonal antibodies Abcam EPR18991 and
Sigma-Aldrich
SAB4200701
were used for detection of Cas9. HRP-conjugated secondary antibodies (GE Healthcare) were used. A mixture
(2)
Integrative characterisation of secreted factors involved in intercellular communication between prostate epithelial or cancer cells and fibroblasts
Molecular Oncology
March 1, 2023
Yunjian Wu, Kimberley C. Clark, Birunthi Niranjan, Anderly C. Chüeh, Lisa G. Horvath, Renea A. Taylor, Roger J. Daly
expressing cell
clones
. Expression of dCas9‐KRAB was validated via
western blot
using an anti‐Cas9 antibody (
SAB4200701
,
Sigma‐Aldrich
). Five pairs of sgRNAs targeting the
human
FST
gene were designed using the Broad Institute's
(3)
High-content image-based CRISPR screening reveals regulators of 3D genome architectures
bioRxiv
February 4, 2023
Mengwei Hu, Bing Yang, Tyler B Jensen, Jonathan S D Radda, Yubao Cheng, Shengyan Jin, Siyuan Wang
were incubated for 1 hour at room temperature with
1:500
diluted anti-Cas9 primary antibody (
Sigma
,
SAB4200701
–25UL, for detection of Cas9 expression) or
1:100
diluted anti-H3K27ac primary antibody (
Active Motif
,
(4)
Hypoxia-directed tumor targeting of CRISPR-Cas9 and HSV-TK suicide gene therapy using lipid nanoparticles
Molecular Therapy - Methods & Clinical Development
June 9, 2022
Alicia Davis, Kevin V. Morris, Galina Shevchenko
sc-47724
), anti-HIF1⍺ (
1:1,000
;
Bethyl Laboratories
A700-001
), anti-spCas9 (
1:10,000
;
Sigma-Aldrich
SAB4200701
), anti-tk (
1:2,000
;
Invitrogen
PA5-67984
), anti-
histone H3
(
1:10
,0000;
Cell Signaling Technologies
14269
),
(5)
Rapid CRISPR/Cas9 Editing of Genotype IX African Swine Fever Virus Circulating in Eastern and Central Africa
Frontiers in Genetics
August 30, 2021
Hussein M. Abkallo et al.
SDS-PAGE
and blotted on nitrocellulose
membrane
. The
mouse
α-
CRISPR/Cas9
mAb (
clone
: 7A9-3A3, Cat. No.
SAB4200701
;
Sigma
), and the β-tubulin specific monoclonal (
T4026
,
Sigma
) were used at dilutions of
1:250
and
1: 1,000
(6)
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B
eLife
May 27, 2021
Fan W, Tang S, Fan X, Fang Y, Xu X, Li L, Xu J, Li JL, Wang Z, Li X.
polyclonal),
Abcam
,
ab8135
; RRID:AB_306298,IF(
1:400
)
Antibody
,
Anti-CRISPR/Cas9 (Mouse monoclonal
),
Millipore Sigma
,
SAB4200701
; RRID:AB_2891217,WB (
1:1000
) Antibody,Anti-Ezh2 (Rabbit monoclonal),
Cell Signaling
,
5246
; RRID:AB_10694683,CHIP(
1:100
)
(7)
Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira
Scientific Reports
December 1, 2019
L. G. V. Fernandes, L. P. Guaman, S. A. Vasconcellos, Marcos B. Heinemann, M. Picardeau, A. L. T. O. Nascimento
PBS containing 0.05%
Tween 20
(PBS-T) and then incubated with
mouse
monoclonal anti-Cas9 antibodies (
SAB4200701
,
1:10,000
in PBS-T containing 1% milk,
Sigma
) for 1 h at room temperature. After washing, detection
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Anti-CRISPR/Cas9 antibody, Mouse monoclonal from MilliporeSigma
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